Oxidative Deamination of S-Adenosyl-L-homocysteine by Rat Kidney L-Amino Acid Oxidase
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منابع مشابه
Oxidative deamination of S-adenosyl-L-homocysteine by rat kidney L-amino acid oxidase.
Cell-free extracts prepared from rat kidney or liver catalyzed the oxidative deamination of S-adenosyl-Lhomocysteine to S-adenosyl-y-thio-a-ketobutyrate. This reaction was found to be catalyzed by L-amino acid oxidase (L-amino acid:oxygen oxidoreductase (deaminating), EC 1.4.3.2). In the presence of catalase, 0.48 pmole of oxygen was consumed for each micromole of substrate oxidized, and 1 pmol...
متن کاملCrystalline mammalian L-amino acid oxidase from rat kidney mitochondria.
L-Amino acid oxidase has been crystallized from extracts of rat kidney mitochondria. Purification involved sonic extraction, ammonium sulfate fractionation, chromatography on diethylaminoethyl cellulose, and gel filtration through Sephadex G-ZOO. The enzyme revealed a single protein band on starch gel electrophoresis and exhibited a single homogeneous peak (a%, W = 10.5 S) in an ultracentrifuge...
متن کاملAmino acid sequence of S-adenosyl-L-homocysteine hydrolase from rat liver as derived from the cDNA sequence.
Rat liver cDNA libraries constructed in lambda gt11 were screened for reactivity with polyclonal antibodies to native S-adenosyl-L-homocysteine (AdoHcy) hydrolase (adenosylhomocysteinase; EC 3.3.1.1). Five clones were isolated and sequenced. The amino acid sequence, deduced from the cDNA sequence, contained the sequence of eight peptides obtained by tryptic and cyanogen bromide fragmentation of...
متن کاملMetabolism of S-adenosyl-L-homocysteine in vivo by the rat.
After intravenous injection of S-adenosyl-L-homocysteine3H (homocysteine-labeled) into rats, less than 15% of the radioisotope was incorporated into protein methionine or excreted in the urine as oc-ketobutyrate. The remaining radioisotope was found associated with an unknown keto acid excreted in the urine. Intravenous injection of Sadenosyl-L-homocysteine-35S or S-adenosyl-3H-L-homocysteine (...
متن کاملcDNA for S-adenosyl-L-homocysteine hydrolase from Catharanthus roseus.
The majority of the Met synthesized in plants is utilized for methylations performed with S-adenosyl-L-Met. These reactions play a major role in the modification of a large variety of acceptor molecules, such as lipids, polysaccharides, nucleic acids, proteins, and secondary plant products (reviewed by Giovanelli, 1987). The hydrolysis of SAH to adenosine and L-homocysteine by SAH hydrolase is ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)94316-4